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proteome profiler human nf κb pathway array  (R&D Systems)


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    R&D Systems proteome profiler human nf κb pathway array
    Proteome Profiler Human Nf κb Pathway Array, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/proteome+profiler+human+nf+%CE%BAb+pathway+array/Proteome+Profiler+Human+NFkB+Pathway+Array/pm41892355-99-14-21
    Average 94 stars, based on 21 article reviews
    proteome profiler human nf κb pathway array - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Membrane:

    Article Title: Modeling Chronic BaP Exposure in Bronchial Epithelial Cells Reveals Multi-Scale Drivers of Early Preneoplastic Reprogramming
    Article Snippet: .. The Proteome Profiler Human Phospho-Kinase Array (ARY003C, R&D Systems, Minneapolis, MN, USA) and the Proteome Profiler Human NF-κB Pathway Array (ARY029, R&D Systems, Minneapolis, MN, USA) are antibody-based membrane arrays in which target proteins are captured by spotted antibodies and detected via biotinylated detection antibodies and streptavidin-conjugated horseradish peroxidase, generating a chemiluminescent signal proportional to target phosphorylation levels. ..

    Article Title: Modeling Chronic BaP Exposure in Bronchial Epithelial Cells Reveals Multi-Scale Drivers of Early Preneoplastic Reprogramming.
    Article Snippet: .. The Proteome Profiler Human Phospho-Kinase Array (ARY003C, R&D Systems, Minneapolis, MN, USA) and the Proteome Profiler Human NF-κB Pathway Array (ARY029, R&D Systems, Minneapolis, MN, USA) are antibody-based membrane arrays in which target proteins are captured by spotted antibodies and detected via biotinylated detection antibodies and streptavidin-conjugated horseradish peroxidase, generating a chemiluminescent signal proportional to target phosphorylation levels. ..

    Phospho-proteomics:

    Article Title: Modeling Chronic BaP Exposure in Bronchial Epithelial Cells Reveals Multi-Scale Drivers of Early Preneoplastic Reprogramming
    Article Snippet: .. The Proteome Profiler Human Phospho-Kinase Array (ARY003C, R&D Systems, Minneapolis, MN, USA) and the Proteome Profiler Human NF-κB Pathway Array (ARY029, R&D Systems, Minneapolis, MN, USA) are antibody-based membrane arrays in which target proteins are captured by spotted antibodies and detected via biotinylated detection antibodies and streptavidin-conjugated horseradish peroxidase, generating a chemiluminescent signal proportional to target phosphorylation levels. ..

    Article Title: Modeling Chronic BaP Exposure in Bronchial Epithelial Cells Reveals Multi-Scale Drivers of Early Preneoplastic Reprogramming.
    Article Snippet: .. The Proteome Profiler Human Phospho-Kinase Array (ARY003C, R&D Systems, Minneapolis, MN, USA) and the Proteome Profiler Human NF-κB Pathway Array (ARY029, R&D Systems, Minneapolis, MN, USA) are antibody-based membrane arrays in which target proteins are captured by spotted antibodies and detected via biotinylated detection antibodies and streptavidin-conjugated horseradish peroxidase, generating a chemiluminescent signal proportional to target phosphorylation levels. ..

    Activation Assay:

    Article Title: Sensitization to Drug Treatment in Precursor B-Cell Acute Lymphoblastic Leukemia Is Not Achieved by Stromal NF-κB Inhibition of Cell Adhesion but by Stromal PKC-Dependent Inhibition of ABC Transporters Activity
    Article Snippet: The analysis was done using the FlowJo Software (v10.0, BD, FlowJo, LLC, Ashland, OR, USA), and with the mean fluorescence intensity (MFI) values, the multidrug resistance activity factor (MAF) was calculated following the instructions provided by the manufacturer. .. The activation of different phospho kinases and the expression of other molecules involved in the regulation of the NF-κB signaling was determined using de Proteome Profiler Human Phospho-Kinase Array (ARY003B, R&D Systems, Minneapolis, MN, USA) and the Proteome Profiler Human NF-κB Pathway Array (ARY029, R&D Systems, Minneapolis, MN, USA) following the instructions of the manufacturer. ..

    Article Title: Simultaneously Targeting Two Coupled Signalling Molecules in the Mesenchymal Stem Cell Support Efficiently Sensitises the Multiple Myeloma Cell Line H929 to Bortezomib.
    Article Snippet: .. Activation of the NF-κB pathway was also evaluated in BM-MSC from co-cultures with H929 cells by using the Proteome Profiler Human NF-κB Pathway Array (ARY029, R&D Systems, Minneapolis, MN, USA) following the instructions of the manufacturer. .. 1 × 106 BM-MSC were seeded in 75 cm2 culture flasks and incubated for 24 h in complete IMDM medium, treated with 20 and 40 μM of ENZA or HKPS for 2 h, and then co-cultured with H929 cells for 2 h. Then, H929 cells were removed by several washes with PBS 1X + EDTA, and BM-MSC were trypsinized for protein extraction and quantitation using the BCA method (Thermo Fisher Scientific, Waltham, MA, USA).

    Expressing:

    Article Title: Sensitization to Drug Treatment in Precursor B-Cell Acute Lymphoblastic Leukemia Is Not Achieved by Stromal NF-κB Inhibition of Cell Adhesion but by Stromal PKC-Dependent Inhibition of ABC Transporters Activity
    Article Snippet: The analysis was done using the FlowJo Software (v10.0, BD, FlowJo, LLC, Ashland, OR, USA), and with the mean fluorescence intensity (MFI) values, the multidrug resistance activity factor (MAF) was calculated following the instructions provided by the manufacturer. .. The activation of different phospho kinases and the expression of other molecules involved in the regulation of the NF-κB signaling was determined using de Proteome Profiler Human Phospho-Kinase Array (ARY003B, R&D Systems, Minneapolis, MN, USA) and the Proteome Profiler Human NF-κB Pathway Array (ARY029, R&D Systems, Minneapolis, MN, USA) following the instructions of the manufacturer. ..

    Transduction:

    Article Title: The Effect of Neddylation Inhibition on Inflammation-Induced MMP9 Gene Expression in Esophageal Squamous Cell Carcinoma
    Article Snippet: .. To determine the relative levels of 41 total and 4 serine/tyrosine phosphorylated proteins involved in NFκB signal transduction, the Proteome Profiler Human NF-κB Pathway Array (R&D Systems, ARY029) was used. ..



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    R&D Systems proteome profiler human nf κb pathway kit
    Figure 5. IL-1α or IL-8 upregulates the NFκB signaling pathway in early MSCs. Early MSCs at 50% confluence were treated with IL-1α (10 ng/mL) or IL-8 (10 ng/mL) in serum-free medium in a 10 cm dish for 24 h, followed by (A) measurement of protein level by <t>proteome</t> profiler human NFκB pathway array kit and (B) quantification of mean pixel intensity by Image J software. The labeled spots are: 1. NFκB1, 2. STAT2, 3. IL-1 RI, 4. NFκB2, 5. IL-17 RA, 6. TNF RI/TNFRSF1A, 7. RelA/p65, 8. FADD/MORT1, 9. IκBε, 10. LTBR/TNFRSF3, 11. SOCS6, 12. STAT1p91, and 13. IKK2/IKKβ. (C) Early MSCs were treated with IL-1α (10 ng/mL) or IL-8 (10 ng/mL) in a 10 cm dish for 72 h, followed by measuring protein levels of IKK2, phospho-serine 536 p65 subunit of NFκB (pS536-p65), p16 and p21 by Western blotting. Actin served as the protein loading control. Fold change to Actin with normalization is shown. Data are expressed as mean ± SD (n = 3). Asterisks indicate significant differences as determined by the unpaired Student’s t-test (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 versus control group, CTR).
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    Image Search Results


    Figure 5. IL-1α or IL-8 upregulates the NFκB signaling pathway in early MSCs. Early MSCs at 50% confluence were treated with IL-1α (10 ng/mL) or IL-8 (10 ng/mL) in serum-free medium in a 10 cm dish for 24 h, followed by (A) measurement of protein level by proteome profiler human NFκB pathway array kit and (B) quantification of mean pixel intensity by Image J software. The labeled spots are: 1. NFκB1, 2. STAT2, 3. IL-1 RI, 4. NFκB2, 5. IL-17 RA, 6. TNF RI/TNFRSF1A, 7. RelA/p65, 8. FADD/MORT1, 9. IκBε, 10. LTBR/TNFRSF3, 11. SOCS6, 12. STAT1p91, and 13. IKK2/IKKβ. (C) Early MSCs were treated with IL-1α (10 ng/mL) or IL-8 (10 ng/mL) in a 10 cm dish for 72 h, followed by measuring protein levels of IKK2, phospho-serine 536 p65 subunit of NFκB (pS536-p65), p16 and p21 by Western blotting. Actin served as the protein loading control. Fold change to Actin with normalization is shown. Data are expressed as mean ± SD (n = 3). Asterisks indicate significant differences as determined by the unpaired Student’s t-test (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 versus control group, CTR).

    Journal: Cells

    Article Title: Paracrine Senescence of Mesenchymal Stromal Cells Involves Inflammatory Cytokines and the NF-κB Pathway.

    doi: 10.3390/cells11203324

    Figure Lengend Snippet: Figure 5. IL-1α or IL-8 upregulates the NFκB signaling pathway in early MSCs. Early MSCs at 50% confluence were treated with IL-1α (10 ng/mL) or IL-8 (10 ng/mL) in serum-free medium in a 10 cm dish for 24 h, followed by (A) measurement of protein level by proteome profiler human NFκB pathway array kit and (B) quantification of mean pixel intensity by Image J software. The labeled spots are: 1. NFκB1, 2. STAT2, 3. IL-1 RI, 4. NFκB2, 5. IL-17 RA, 6. TNF RI/TNFRSF1A, 7. RelA/p65, 8. FADD/MORT1, 9. IκBε, 10. LTBR/TNFRSF3, 11. SOCS6, 12. STAT1p91, and 13. IKK2/IKKβ. (C) Early MSCs were treated with IL-1α (10 ng/mL) or IL-8 (10 ng/mL) in a 10 cm dish for 72 h, followed by measuring protein levels of IKK2, phospho-serine 536 p65 subunit of NFκB (pS536-p65), p16 and p21 by Western blotting. Actin served as the protein loading control. Fold change to Actin with normalization is shown. Data are expressed as mean ± SD (n = 3). Asterisks indicate significant differences as determined by the unpaired Student’s t-test (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 versus control group, CTR).

    Article Snippet: Early MSCs until reaching 80% confluence were incubated with 10 ng/mL IL-1α or 10 ng/mL IL-8 recombinants (Peprotech, Rocky Hill, NJ, USA), respectively, in serum-free DMEM supplemented with 1% antibiotics for 24 h. The protein expression analysis of early MSCs and pro-inflammatory activation were performed using a Proteome Profiler Human NF-κB Pathway Kit (ARY029, R&D Systems, Minneapolis, MN, USA).

    Techniques: Software, Labeling, Western Blot, Control